Publications

2024

ALS-associated FUS mutation reshapes the RNA and protein composition of Stress Granules Davide Mariani, Adriano Setti, Francesco Castagnetti, Erika Vitiello, Lorenzo Stufera Mecarelli, Gaia di Timoteo, Andrea Giuliani, *Eleonora Perego*, *Sabrina Zappone*, Nara Liessi, Andrea Armirotti, *Giuseppe Vicidomini*, Irene Bozzoni Published in bioRxiv, January 2024 (see publication ) - Paper

Compact and effective photon-resolved image scanning microscope *Giorgio Tortarolo*, *Alessandro Zunino*, *Simonluca Piazza*, *Mattia Donato*, *Sabrina Zappone*, Agnieszka Pierzyńska-Mach, *Marco Castello*, *Giuseppe Vicidomini* Published in Adv. Phot., January 2024 (see publication ) - Paper, Corresponding author

2023

Single-photon microscopy to study biomolecular condensates *Eleonora Perego*, *Sabrina Zappone*, Francesco Castagnetti, Davide Mariani, Erika Vitiello, Jakob Rupert, Elsa Zacco, Gian Gaetano Tartaglia, Irene Bozzoni, *Eli Slenders*, *Giuseppe Vicidomini * Published in Nat. Comm., December 2023 (see publication ) - Paper, Corresponding author

M6A reduction relieves FUS-associated ALS granules Gaia Di Timoteo, Andrea Giuliani, Adriano Setti, Martina C. Biagi, Michela Lisi, Alessia Grandioso, Davide Mariani, Francesco Castagnetti, *Eleonora Perego*, *Sabrina Zappone*, *Giuseppe Vicidomini*, Dante Rotili, Mario Sabatelli, Serena Lattante, Irene Bozzoni Published in bioRxiv, October 2023 (see publication ) - Paper

4D Single-Particle Tracking with Asynchronous Read-Out SPAD-Array Detector *Andrea Bucci*, *Giorgio Tortarolo*, *Marcus Oliver Held*, *Luca Bega*, *Eleonora Perego*, Francesco Castagnetti, Irene Bozzoni, *Eli Slenders*, *Giuseppe Vicidomini* Published in bioRxiv, August 2023 (see publication ) - Paper, Corresponding author

Image scanning microscopy with a doughnut beam: signal strength and integrated intensity Colin J.R. Sheppard, *Marco Castello*, *Giorgio Tortarolo*, *Alessandro Zunino*, *Eli Slenders*, Paolo Bianchini, *Giuseppe Vicidomini*, Alberto Diaspro Published in J. Opt. Soc. Am. A, August 2023 (see publication ) - Paper

Compact and effective photon-resolved image scanning microscope *Giorgio Tortarolo*, *Alessandro Zunino*, *Simonluca Piazza*, *Mattia Donato*, *Sabrina Zappone*, Agnieszka Pierzyńska-Mach, *Marco Castello*, *Giuseppe Vicidomini* Published in bioRxiv, July 2023 (see publication ) - Paper, Corresponding author

Content-enriched fluorescence lifetime fluctuation spectroscopy to study bio-molecular condensate formation *Eleonora Perego*, *Sabrina Zappone*, Francesco Castagnetti, Davide Mariani, Erika Vitiello, Jakob Rupert, Elsa Zacco, Gian Gaetano Tartaglia, Irene Bozzoni, *Eli Slenders*, *Giuseppe Vicidomini* Published in bioRxiv, June 2023 (see publication ) - Paper, Corresponding author

Background Rejection in Two-Photon Fluorescence Image Scanning Microscopy Colin J.R. Sheppard, *Marco Castello*, *Giorgio Tortarolo*, *Alessandro Zunino*, *Eli Slenders*, Paolo Bianchini, *Giuseppe Vicidomini*, Alberto Diaspro Published in Photonics, May 2023 (see publication ) - Paper

Open-source tools enable accessible and advanced image scanning microscopy data analysis *Alessandro Zunino*, *Eli Slenders*, *Francesco Fersini*, *Andrea Bucci*, *Mattia Donato*, *Giuseppe Vicidomini* Published in Nat. Photon., May 2023 (see publication ) - Paper, Corresponding author

Reconstructing the Image Scanning Microscopy Dataset: an Inverse Problem *Alessandro Zunino*, *Marco Castello*, *Giuseppe Vicidomini* Published in Inverse Probl., April 2023 (see publication ) - Paper, Corresponding author

ISM-FLUX: MINFLUX with an array detector *Eli Slenders*, *Giuseppe Vicidomini* Published in Phys. Rev. Research, April 2023 (see publication ) - Paper, Corresponding author

SPLIT-PIN software enabling confocal and super-resolution imaging with a virtually closed pinhole Elisabetta Di Franco, Angelita Costantino, Elena Cerutti, Morgana D’Amico, Anna P Privitera, Paolo Bianchini, *Giuseppe Vicidomini*, Massimo Gulisano, Alberto Diaspro, Luca Lanzanò Published in Sci. Rep., March 2023 (see publication ) - Paper

2022

Signal strength and integrated intensity in confocal and image scanning microscopy Colin J.R. Sheppard, *Marco Castello*, *Giorgio Tortarolo*, *Alessandro Zunino*, *Eli Slenders*, Paolo Bianchini, *Giuseppe Vicidomini*, Alberto Diaspro Published in J. Opt. Soc. Am. A, December 2022 (see publication ) - Paper

Focus image scanning microscopy for sharp and gentle super-resolved microscopy *Giorgio Tortarolo*, *Alessandro Zunino*, *Francesco Fersini*, *Marco Castello*, *Simonluca Piazza*, Colin J.R. Sheppard, Paolo Bianchini, Alberto Diaspro, *Sami V. Koho*, *Giuseppe Vicidomini* Published in Nat. Comm., December 2022 (see publication ) - Paper, Corresponding author

The BrightEyes-TTM as an open-source time-tagging module for democratising single-photon microscopy *Alessandro Rossetta*, *Eli Slenders*, *Mattia Donato*, *Sabrina Zappone*, *Francesco Fersini*, Martina Bruno, Francesco Diotalevi, Luca Lanzanò, *Sami V. Koho*, *Giorgio Tortarolo*, Andrea Barberis, Marco Crepaldi, *Eleonora Perego*, *Giuseppe Vicidomini* Published in Nat. Comm., December 2022 (see publication ) - Paper, Corresponding author

Reconstructing the Image Scanning Microscopy Dataset: an Inverse Problem *Alessandro Zunino*, *Marco Castello*, *Giuseppe Vicidomini* Published in arXiv, November 2022 (see publication ) - Preprint, Corresponding author

Focus-ISM for Sharp and Gentle Super-Resolved Microscopy *Giorgio Tortarolo*, *Alessandro Zunino*, *Francesco Fersini*, *Marco Castello*, *Simonluca Piazza*, Colin J.R. Sheppard, Paolo Bianchini, Alberto Diaspro, *Sami V. Koho*, *Giuseppe Vicidomini* Published in bioRxiv, April 2022 (see publication ) - Paper, Corresponding author

ISM-FLUX: single-step MINFLUX with an array detector *Eli Slenders*, *Giuseppe Vicidomini* Published in bioRxiv, April 2022 (see publication ) - Paper, Corresponding author

2021

The BrightEyes-TTM: an Open-Source Time-Tagging Module for Single-Photon Microscopy *Alessandro Rossetta*, *Eli Slenders*, *Mattia Donato*, *Eleonora Perego*, Francesco Diotalevi, Luca Lanzanó, *Sami V. Koho*, *Giorgio Tortarolo*, Marco Crepaldi, *Giuseppe Vicidomini* Published in bioRxiv, October 2021 (see publication ) - Paper, Corresponding author

Cooled SPAD array detector for low light-dose fluorescence laser scanning microscopy *Eli Slenders*, *Eleonora Perego*, Mauro Buttafava, *Giorgio Tortarolo*, Enrico Conca, *Sabrina Zappone*, Agnieszka Pierzynska-Mach, Federica Villa, Enrica Maria Petrini, Andrea Barberis, Alberto Tosi, *Giuseppe Vicidomini* Published in Biophys. Rep., September 2021 (see publication ) - Paper, Corresponding author

Cooled SPAD array detector for low light-dose fluorescence laser scanning microscopy *Eli Slenders*, *Eleonora Perego*, Mauro Buttafava, *Giorgio Tortarolo*, Enrico Conca, *Sabrina Zappone*, Agnieszka Pierzynska-Mach, Federica Villa, Enrica Maria Petrini, Andrea Barberis, Alberto Tosi, *Giuseppe Vicidomini* Published in bioRxiv, August 2021 (see publication ) - Paper, Corresponding author

Evaluation of STED super-resolution image quality by image correlation spectroscopy (QuICS) Elena Cerutti, Morgana D'Amico, Isotta Cainero, Gaetano Ivan Dellino, Mario Faretta, *Giuseppe Vicidomini*, Pier Giuseppe Pelicci, Paolo Bianchini, Alberto Diaspro Published in bioRxiv, August 2021 (see publication ) - Paper

Evaluation of STED super-resolution image quality by image correlation spectroscopy (QuICS) Elena Cerutti, Morgana D'Amico, Isotta Cainero, Gaetano Ivan Dellino, Mario Faretta, *Giuseppe Vicidomini*, Pier Giuseppe Pelicci, Paolo Bianchini, Alberto Diaspro Published in Sci. Rep., August 2021 (see publication ) - Paper

PRRT2 modulates presynaptic Ca2+ influx by interacting with P/Q-type channels Daniele Ferrante, Bruno Sterlini, Cosimo Prestigio, Antonella Marte, Anna Corradi, Franco Onofri, *Giorgio Tortarolo*, *Giuseppe Vicidomini*, Andrea Petretto, Jessica Muià, Agnes Thalhammer, Pierluigi Valente, Lorenzo A Cingolani, Fabio Benfenati, Pietro Baldelli Published in Cell Rep., June 2021 (see publication ) - Paper

Chromatin investigation in the nucleus using a phasor approach to structured illumination microscopy Isotta Cainero, Elena Cerutti, Mario Faretta, Gaetano Ivan Dellino, Pier Giuseppe Pelicci, Paolo Bianchini, *Giuseppe Vicidomini*, Alberto Diaspro, Luca Lanzanò Published in Biophys. J., May 2021 (see publication ) - Paper

Confocal-based fluorescence fluctuation spectroscopy with a SPAD array detector *Eli Slenders*, *Marco Castello*, Mauro Buttafava, Federica Villa, Alberto Tosi, Luca Lanzano, *Sami V. Koho*, *Giuseppe Vicidomini* Published in Light Sci. Appl., February 2021 (see publication ) - Paper, Corresponding author

Pixel reassignment in image scanning microscopy with a doughnut beam: example of maximum likelihood restoration Colin J.R. Sheppard, *Marco Castello*, *Giorgio Tortarolo*, *Eli Slenders*, Takahiro Deguchi, *Sami V. Koho*, Paolo Bianchini, *Giuseppe Vicidomini*, Alberto Diaspro Published in J. Opt. Soc. Am. A, January 2021 (see publication ) - Paper

2020

Image scanning microscopy with multiphoton excitation or Bessel beam illumination Colin J. R. Sheppard, *Marco Castello*, *Giorgio Tortarolo*, *Eli Slenders*, Takahiro Deguchi, *Sami V. Koho*, *Giuseppe Vicidomini*, Alberto Diaspro Published in J. Opt. Soc. Am. A, September 2020 (see publication ) - Paper

SPAD-based asynchronous-readout array detectors for image-scanning microscopy Mauro Buttafava, Federica Villa, *Marco Castello*, *Giorgio Tortarolo*, Enrico Conca, Mirko Sanzaro, *Simonluca Piazza*, Paolo Bianchini, Alberto Diaspro, Franco Zappa, *Giuseppe Vicidomini*, Alberto Tosi Published in Optica, July 2020 (see publication ) - Paper

Two-photon image-scanning microscopy with SPAD array and blind image reconstruction *Sami V. Koho*, *Eli Slenders*, *Giorgio Tortarolo*, *Marco Castello*, Mauro Buttafava, Federica Villa, Elena Tcarenkova, Marcel Ameloot, Paolo Bianchini, Colin J.R. Sheppard, Albeto Diaspro, Alberto Tosi, *Giuseppe Vicidomini* Published in Biomed. Opt. Express, May 2020 (see publication ) - Paper, Corresponding author

Improving SPLIT-STED Super-Resolution Imaging with Tunable Depletion and Excitation Power Simone Pelicci, *Giorgio Tortarolo*, *Giuseppe Vicidomini*, Alberto Diaspro, Luca Lanzanò Published in J. Phys. D, March 2020 (see publication ) - Paper

SPAD-based asynchronous-readout array detectors for image-scanning microscopy Mauro Buttafava, Federica Villa, *Marco Castello*, *Giorgio Tortarolo*, Enrico Conca, Mirko Sanzaro, *Simonluca Piazza*, Paolo Bianchini, Alberto Diaspro, Franco Zappa, *Giuseppe Vicidomini*, Alberto Tosi Published in arXiv, February 2020 (see publication ) - Preprint

2019

Pixel reassignment in image scanning microscopy: a re-evaluation Colin J. R. Sheppard, *Marco Castello*, *Giorgio Tortarolo*, Takahiro Deguchi, *Sami V. Koho*, *Giuseppe Vicidomini*, Alberto Diaspro Published in J. Opt. Soc. Am. A, December 2019 (see publication ) - Paper

Efficient Multiphoton STED Nanoscope Exploiting Spatio-Temporal Information *Ivan Coto Hernandez*, *Marco Castello*, *Giorgio Tortarolo*, Nathan Jowett, Alberto Diaspro, Luca Lanzanò, *Giuseppe Vicidomini* Published in Neurophotonics, November 2019 (see publication ) - Paper, Corresponding author

Photon-separation to enhance the spatial resolution of pulsed STED microscopy *Giorgio Tortarolo*, Yuansheng Sun, Kai Wen Teng, Yuji Ishitsuka, Luca Lanzanó, Paul R. Selvin, Beniamino Barbieri, Alberto Diaspro, *Giuseppe Vicidomini* Published in Nanoscale, September 2019 (see publication ) - Paper, Corresponding author

Synergic Combination of Stimulated Emission Depletion Microscopy with Image Scanning Microscopy to Reduce Light Dosage *Giorgio Tortarolo*, *Marco Castello*, *Sami V. Koho*, *Giuseppe Vicidomini* Published in bioRxiv, August 2019 (see publication ) - Preprint, Corresponding author

Fourier ring correlation simplifies image restoration in fluorescence microscopy *Sami V. Koho*, *Giorgio Tortarolo*, *Marco Castello*, Talahiro Deguchi, Albeto Diaspro, *Giuseppe Vicidomini* Published in Nat. Comm., July 2019 (see publication ) - Paper, Corresponding author

Easy Two-Photon Image-Scanning Microscopy With Spad Array And Blind Image Reconstruction *Sami V. Koho*, *Eli Slenders*, *Giorgio Tortarolo*, *Marco Castello*, Mauro Buttafava, Federica Villa, Elena Tcarenkova, Marcel Ameloot, Paolo Bianchini, Colin J.R. Sheppard, Albeto Diaspro, Alberto Tosi, *Giuseppe Vicidomini* Published in bioRxiv, April 2019 (see publication ) - Preprint, Corresponding author

A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM *Marco Castello*, *Giorgio Tortarolo*, Mauro Buttafava, Takahiro Deguchi, Federica Villa, Sami Koho, Luca Pesce, Michele Oneto, Simone Pelicci, Luca Lanzanó, Paolo Bianchini, Colin J. R. Sheppard, Alberto Diaspro, Alberto Tosi, *Giuseppe Vicidomini* Published in Nat. Methods, January 2019 (see publication ) - Paper, Corresponding author

2018

STED super-resolved microscopy *Giuseppe Vicidomini*, Paolo Bianchini, Alberto Diaspro Published in Nat. Methods, January 2018 (see publication ) - Review, Corresponding author

2015

Encoding and decoding spatio-temporal information for super-resolution microscopy Luca Lanzanò, Ivan Coto Hernández, *Marco Castello*, Enrico Gratton, Alberto Diaspro, *Giuseppe Vicidomini * Published in Nat. Comm., April 2015 (see publication ) - Paper, Corresponding author