Eleonora Perego
Fluorescence Fluctuation Spectroscopy
Started on January, 2021
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Short Bio
Eleonora Perego studied Physics at the University of Milano-Bicocca (Italy). She got a M. Sc. in Physics in 2015, after a period at the AMOLF Institute(Amsterdam, the Netherlands), working in the group of “Developmental Biology” of Dr. Jeroen van Zon. In 2020 she obtained a PhD in Biophysics, titled “Studying molecular interaction under flow with fluorescence fluctuation spectroscopy”, working in the group of “Cellular Biophysics” of Prof. Dr. Sarah Koester (Göttingen, Germany), where she studied with fluorescence spectroscopy methods combined with microfluidic approaches protein-protein interactions.
Since 2021, Eleonora is working at the Italian Institute of Technology in Genoa (Italy) in the Molecular Microscopy and Spectroscopy research line of Dr. Giuseppe Vicidomini as a post-doctoral researcher. Her research interests include the application of fluorescence spectroscopy methods, such as fluorescence fluctuation spectroscopy and fluorescence lifetime imaging, to biological questions, ranging from neuroscience to RNA biology.
Projects Description
SPAD-FLFS: In this project, we employ the enrich-information dataset provided by a 5x5 cooled SPAD array detector implemented into a laser-scanning microsope to perform multimodal fluorescence fluctuation analysis on biological samples. In combination with a fast and flexible data-acquisition card developed in our group (the BrightEyes-TTM platform), and a pulsed-laser, each pixel of the detector can register the arrival time of a photon with sub-nanosecond temporal resolution. Thus, this setup can simultaneously be employed to perform imaging, fluorescence fluctation spectroscopy and fluorescence lifetime, opening up several new ways of accurately study biological processes. In particular, we combine fluorescence spectroscopy methods, such as spot-variation FCS, fluorescence lifetime or image-correlation spectroscopy, in order to investigate the dynamics of biological molecules in combination with structural changes and cellular functions. This, will help to elucidate several molecular processes such as the ones happening during RNA-regulation and synaptic plasticity formation.
Publications with our group (see more on Google Scholar)
| ALS-associated FUS mutation reshapes the RNA and protein composition of Stress Granules Davide Mariani, Adriano Setti, Francesco Castagnetti, Erika Vitiello, Lorenzo Stufera Mecarelli, Gaia di Timoteo, Andrea Giuliani, Eleonora Perego, Sabrina Zappone, Nara Liessi, Andrea Armirotti, Giuseppe Vicidomini, Irene Bozzoni Published in bioRxiv, January 2024 (see publication ) |
| Single-photon microscopy to study biomolecular condensates Eleonora Perego, Sabrina Zappone, Francesco Castagnetti, Davide Mariani, Erika Vitiello, Jakob Rupert, Elsa Zacco, Gian Gaetano Tartaglia, Irene Bozzoni, Eli Slenders, Giuseppe Vicidomini Published in Nat. Comm., December 2023 (see publication ) |
| M6A reduction relieves FUS-associated ALS granules Gaia Di Timoteo, Andrea Giuliani, Adriano Setti, Martina C. Biagi, Michela Lisi, Alessia Grandioso, Davide Mariani, Francesco Castagnetti, Eleonora Perego, Sabrina Zappone, Giuseppe Vicidomini, Dante Rotili, Mario Sabatelli, Serena Lattante, Irene Bozzoni Published in bioRxiv, October 2023 (see publication ) |
| 4D Single-Particle Tracking with Asynchronous Read-Out SPAD-Array Detector Andrea Bucci, Giorgio Tortarolo, Marcus Oliver Held, Luca Bega, Eleonora Perego, Francesco Castagnetti, Irene Bozzoni, Eli Slenders, Giuseppe Vicidomini Published in bioRxiv, August 2023 (see publication ) |
| Content-enriched fluorescence lifetime fluctuation spectroscopy to study bio-molecular condensate formation Eleonora Perego, Sabrina Zappone, Francesco Castagnetti, Davide Mariani, Erika Vitiello, Jakob Rupert, Elsa Zacco, Gian Gaetano Tartaglia, Irene Bozzoni, Eli Slenders, Giuseppe Vicidomini Published in bioRxiv, June 2023 (see publication ) |
| The BrightEyes-TTM as an open-source time-tagging module for democratising single-photon microscopy Alessandro Rossetta, Eli Slenders, Mattia Donato, Sabrina Zappone, Francesco Fersini, Martina Bruno, Francesco Diotalevi, Luca Lanzanò, Sami V. Koho, Giorgio Tortarolo, Andrea Barberis, Marco Crepaldi, Eleonora Perego, Giuseppe Vicidomini Published in Nat. Comm., December 2022 (see publication ) |
| The BrightEyes-TTM: an Open-Source Time-Tagging Module for Single-Photon Microscopy Alessandro Rossetta, Eli Slenders, Mattia Donato, Eleonora Perego, Francesco Diotalevi, Luca Lanzanó, Sami V. Koho, Giorgio Tortarolo, Marco Crepaldi, Giuseppe Vicidomini Published in bioRxiv, October 2021 (see publication ) |
| Cooled SPAD array detector for low light-dose fluorescence laser scanning microscopy Eli Slenders, Eleonora Perego, Mauro Buttafava, Giorgio Tortarolo, Enrico Conca, Sabrina Zappone, Agnieszka Pierzynska-Mach, Federica Villa, Enrica Maria Petrini, Andrea Barberis, Alberto Tosi, Giuseppe Vicidomini Published in Biophys. Rep., September 2021 (see publication ) |
| Cooled SPAD array detector for low light-dose fluorescence laser scanning microscopy Eli Slenders, Eleonora Perego, Mauro Buttafava, Giorgio Tortarolo, Enrico Conca, Sabrina Zappone, Agnieszka Pierzynska-Mach, Federica Villa, Enrica Maria Petrini, Andrea Barberis, Alberto Tosi, Giuseppe Vicidomini Published in bioRxiv, August 2021 (see publication ) |